Human EEF1E1 ELISA Kit

Starting at: $696.00

  • Model: ELI-16382h
  • 20 Units in Stock
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Human Eukaryotic translation elongation factor 1 epsilon- 1, EEF1E1 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

EEF1E1,AIMP3; P18; ARS-interacting multifunctional protein 3; aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; eukaryotic translation elongation factor 1 epsilon-1; multisynthase complex auxiliary component p18; p18 component of aminoacyl-tRNA synthetase complex; eukaryotic translation elongation factor 1 epsilon 1,Aminoacyl tRNA synthetase complex-int

 

Search name

Human EEF1E1 ELISA KIT ,Human AIMP3 ELISA KIT ,Human P18 ELISA KIT ,Human ARS-interacting multifunctional protein 3 ELISA KIT ,Human aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 ELISA KIT ,Human eukaryotic translation elongation factor 1 epsilon-1 ELISA KIT ,Human multisynthase complex auxiliary component p18 ELISA KIT ,Human p18 component of aminoacyl-tRNA synthetase complex ELISA KIT ,Human eukaryotic translation elongation factor 1 epsilon 1 ELISA KIT ,Human Aminoacyl tRNA synthetase complex-int ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Eukaryotic translation elongation factor 1 epsilon- 1, EEF1E1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EEF1E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EEF1E1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EEF1E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EEF1E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

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