Human EIF3F ELISA KIT

Product Name:Human EIF3F ELISA KIT
Packing:96T

Catalog No.:ELI-26101h

Gene Name:EIF3F

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Human EIF3F

Alternative Name:EIF3F,Human EIF3F
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human EIF3F ELISA KIT allows for the in vitro quantitative determination of Human EIF3F concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human EIF3F ELISA KIT has been pre-coated with an Human EIF3F antibody specific to Human EIF3F .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human EIF3F and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human EIF3F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human EIF3F in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!