Human Endothelial nitric oxide synthase ELISA Kit

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  • Model: ELA-E0868h
  • 20 Units in Stock
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Human Endothelial NOS, eNOS ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Nitric oxide synthase, endothelial,NOS type III(NOSIII),Endothelial NOS(eNOS),Constitutive NOS(cNOS),EC-NOS, nitric oxide synthase,
endothelial; nitric oxide synthase 3 (endothelial cell)

Search name
Human Endothelial nitric oxide synthase 3 ELISA Kit, Human eNOS-3 ELISA Kit, Human Nitric oxide synthase endothelial ELISA
Kit, Human NOS type III ELISA Kit, Human NOSIII ELISA Kit, Human Endothelial NOS ELISA Kit, Human eNOS ELISA Kit, Human
Constitutive NOS ELISA Kit, Human cNOS ELISA Kit, Human EC-NOS ELISA Kit, Human EC-NOS ELISA Kit, Human nitric oxide
synthase 3 ELISA Kit, Human endothelial cell ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human nitric oxide synthase, endothelial,ENOS concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric oxide synthase, endothelial. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Nitric
oxide synthase, endothelial and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Nitric oxide synthase, endothelial, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Nitric oxide synthase, endothelial in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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