Human EPX ELISA Kit
Product Name:Human EPX ELISA Kit
Packing:96T
Catalog No.:ELA-E8699h
Gene Name:EPX
Detect Range:78-5000 pg/mL
Sensitivity:34pg/mL
Target Protein Name:Human Eosinophil peroxidase
Alternative Name:Eosinophil peroxidase, EPO, EPX, EPER, EPP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human EPX ELISA Kit allows for the in vitro quantitative determination of Human Eosinophil peroxidase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human EPX ELISA Kit has been pre-coated with an EPX antibody specific to Human Eosinophil peroxidase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Eosinophil peroxidase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Eosinophil peroxidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Eosinophil peroxidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!