Human ERBB2 ELISA Kit

Product Name:Human ERBB2 ELISA Kit
Packing:96T

Catalog No.:ELA-E1867h

Gene Name:ERBB2

Detect Range:0.312-20 ng/mL

Sensitivity:0.1ng/mL

Target Protein Name:Human Receptor tyrosine-protein kinase erbB-2

Alternative Name:sp185,HER-2,neu,c-erbB-2,Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ERBB2 ELISA Kit allows for the in vitro quantitative determination of Human Receptor tyrosine-protein kinase erbB-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ERBB2 ELISA Kit has been pre-coated with an ERBB2 antibody specific to Human Receptor tyrosine-protein kinase erbB-2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Receptor tyrosine-protein kinase erbB-2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Receptor tyrosine-protein kinase erbB-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Receptor tyrosine-protein kinase erbB-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!