Human fatty acid- binding protein ELISA Kit

Product Name:Human fatty acid- binding protein ELISA Kit
Packing:96T

Catalog No.:ELA-E1277h

Gene Name:FABP

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:fatty acid-binding protein

Alternative Name:FABP,fatty acid-binding protein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human fatty acid- binding protein ELISA Kit allows for the in vitro quantitative determination of fatty acid-binding protein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human fatty acid- binding protein ELISA Kit has been pre-coated with an fatty acid-binding protein antibody specific to fatty acid-binding protein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for fatty acid-binding protein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain fatty acid-binding protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of fatty acid-binding protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!