Human FGF8 ELISA Kit

Product Name:Human FGF8 ELISA Kit
Packing:96T

Catalog No.:ELA-E2137h

Gene Name:FGF8

Detect Range:15.6-1000 pg/mL

Sensitivity:7.80 pg/mL

Target Protein Name:Human Fibroblast growth factor 8

Alternative Name:Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, FGF8, AIGF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human FGF8 ELISA Kit allows for the in vitro quantitative determination of Human Fibroblast growth factor 8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human FGF8 ELISA Kit has been pre-coated with an FGF8 antibody specific to Human Fibroblast growth factor 8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Fibroblast growth factor 8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Fibroblast growth factor 8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Fibroblast growth factor 8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!