Human Fibroblast growth factor 19, FGF19 ELISA KIT

Human Fibroblast growth factor 19, FGF19 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human FGF19 ELISA KIT ,Human FGF-19 ELISA KIT ,Human fibroblast growth factor 19 ELISA KIT

Intended use

Human FGF19 ELISA kit allows for the in vitro quantitative determination of Human FGF19 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Human FGF19 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FGF19 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FGF19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FGF19 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human FGF19 ELISA kit range 15.6-1000pg/ml

Human FGF19 ELISA kit manual download

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