Human Fibronectin type III domain- containing protein 5, FNDC5 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
FNDC5,FRCP2; irisin; fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2; fibronectin type III domain containing 5
Search name
Human FNDC5 ELISA KIT ,Human FRCP2 ELISA KIT ,Human irisin ELISA KIT ,Human fibronectin type III domain-containing protein 5 ELISA KIT ,Human fibronectin type III repeat-containing protein 2 ELISA KIT ,Human fibronectin type III domain containing 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human fibronectin type iii domain-containing protein 5,FNDC5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to FNDC5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FNDC5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FNDC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FNDC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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