Human FOXC2 ELISA Kit

Product Name:Human FOXC2 ELISA Kit
Packing:96T

Catalog No.:ELA-E12449h

Gene Name:FOXC2

Detect Range:0.156-10 ng/mL

Sensitivity:0.056ng/ml

Target Protein Name:Human Forkhead box protein C2

Alternative Name:Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, MFH-1 protein, Transcription factor FKH-14, FOXC2, FKHL14, MFH1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human FOXC2 ELISA Kit allows for the in vitro quantitative determination of Human Forkhead box protein C2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human FOXC2 ELISA Kit has been pre-coated with an FOXC2 antibody specific to Human Forkhead box protein C2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Forkhead box protein C2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Forkhead box protein C2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Forkhead box protein C2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!