Human FZD1 ELISA Kit

Product Name:Human FZD1 ELISA Kit
Packing:96T

Catalog No.:ELA-E11110h

Gene Name:FZD1

Detect Range:0.78-50 ng/mL

Sensitivity:0.34 ng/ml

Target Protein Name:Human Frizzled-1

Alternative Name:Frizzled-1, Fz-1, hFz1, FzE1, FZD1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human FZD1 ELISA Kit allows for the in vitro quantitative determination of Human Frizzled-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human FZD1 ELISA Kit has been pre-coated with an FZD1 antibody specific to Human Frizzled-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Frizzled-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Frizzled-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Frizzled-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!