Human Galectin 8 ELISA Kit

Product Name:Human Galectin 8 ELISA Kit
Packing:96T

Catalog No.:ELA-E1108h

Gene Name:GAL8

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Galectin 8

Alternative Name:GAL8,Galectin 8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Galectin 8 ELISA Kit allows for the in vitro quantitative determination of Galectin 8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Galectin 8 ELISA Kit has been pre-coated with an Galectin 8 antibody specific to Galectin 8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Galectin 8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Galectin 8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Galectin 8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!