Human GBP5 (Guanylate- binding protein 5) ELISA Kit

96 Tests

Operating instructions                                                              Manual

FOR RESEARCH USE ONLY!

Alternative name

Human GBP5 ELISA KIT ,Human GBP-5 ELISA KIT ,Human GBP-TA antigen ELISA KIT ,Human GTP-binding protein 5 ELISA KIT ,Human guanine nucleotide-binding protein 5 ELISA KIT ,Human guanylate binding protein 5 ELISA KIT

Intended use

Human GBP5 ELISA kit allows for the in vitro quantitative determination of Human Guanylate- binding protein 5 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Human GBP5 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GBP5, During the reaction, GBP5 in the sample or standard competes with a fixed amount of biotin-labeled GBP5 for sites on a pre-coated Monoclonal antibody specific to GBP5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GBP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human GBP5 ELISA kit Range 125-8000pg/ml

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