Human GDF15 ELISA Kit

Product Name:Human GDF15 ELISA Kit
Packing:96T

Catalog No.:ELA-E2034h

Gene Name:GDF15

Detect Range:15.6-1000 pg/mL

Sensitivity:7.8pg/mL

Target Protein Name:Human Growth/differentiation factor 15

Alternative Name:Growth/differentiation factor 15, GDF-15, Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human GDF15 ELISA Kit allows for the in vitro quantitative determination of Human Growth/differentiation factor 15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human GDF15 ELISA Kit has been pre-coated with an GDF15 antibody specific to Human Growth/differentiation factor 15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Growth/differentiation factor 15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Growth/differentiation factor 15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Growth/differentiation factor 15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!