Human GDNF family receptor alpha- 4, GFRA4 ELISA KIT
Product Name:Human GDNF family receptor alpha- 4, GFRA4 ELISA KIT
Packing:96T
Catalog No.:ELI-21480h
Gene Name:GFRA4
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:GDNF family receptor alpha-4(GFRA4
Alternative Name:GFRA4,GDNF family receptor alpha-4(GFRA4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human GDNF family receptor alpha- 4, GFRA4 ELISA KIT allows for the in vitro quantitative determination of GDNF family receptor alpha-4(GFRA4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human GDNF family receptor alpha- 4, GFRA4 ELISA KIT has been pre-coated with an GDNF family receptor alpha-4(GFRA4 antibody specific to GDNF family receptor alpha-4(GFRA4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GDNF family receptor alpha-4(GFRA4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GDNF family receptor alpha-4(GFRA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of GDNF family receptor alpha-4(GFRA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!