Human Glucokinase regulatory protein, GCKR ELISA KIT

Product Name:Human Glucokinase regulatory protein, GCKR ELISA KIT
Packing:96T

Catalog No.:ELI-04492h

Gene Name:GCKR

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Glucokinase regulatory protein(GCKR

Alternative Name:GCKR,Glucokinase regulatory protein(GCKR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Glucokinase regulatory protein, GCKR ELISA KIT allows for the in vitro quantitative determination of Glucokinase regulatory protein(GCKR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Glucokinase regulatory protein, GCKR ELISA KIT has been pre-coated with an Glucokinase regulatory protein(GCKR antibody specific to Glucokinase regulatory protein(GCKR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glucokinase regulatory protein(GCKR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glucokinase regulatory protein(GCKR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Glucokinase regulatory protein(GCKR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!