Human Granzyme K, GZMK ELISA KIT

Product Name:Human Granzyme K, GZMK ELISA KIT
Packing:96T

Catalog No.:ELI-39081h

Gene Name:GZMK

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Granzyme K(GZMK

Alternative Name:GZMK,Granzyme K(GZMK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Granzyme K, GZMK ELISA KIT allows for the in vitro quantitative determination of Granzyme K(GZMK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Granzyme K, GZMK ELISA KIT has been pre-coated with an Granzyme K(GZMK antibody specific to Granzyme K(GZMK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Granzyme K(GZMK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Granzyme K(GZMK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Granzyme K(GZMK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!