Human Growth differentiation factor 15, GDF15 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

GDF15,GDF-15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PTGF-beta; macrophage inhibitory cytokine 1; placental TGF-beta; placental bone morphogenetic protein; prostate differentiation factor; growth differentiation factor 15

Search name

Human GDF15 ELISA Kit ,Human GDF-15 ELISA Kit ,Human MIC-1 ELISA Kit ,Human MIC1 ELISA Kit ,Human NAG-1 ELISA Kit ,Human PDF ELISA Kit ,Human PLAB ELISA Kit ,Human PTGFB ELISA Kit ,Human NRG-1 ELISA Kit ,Human NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1 ELISA Kit ,Human NSAID-activated gene 1 protein ELISA Kit ,Human NSAID-regulated gene 1 protein ELISA Kit ,Human PTGF-beta ELISA Kit ,Human macrophage inhibitory cytokine 1 ELISA Kit ,Human placental TGF-beta ELISA Kit ,Human placental bone morphogenetic protein ELISA Kit ,Human prostate differentiation factor ELISA Kit ,Human growth differentiation factor 15 ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human growth/differentiation factor 15,GDF-15 concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF-15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GDF-15 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GDF-15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GDF-15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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