Human HDL- cholesterol, HDLC ELISA KIT

Product Name:Human HDL- cholesterol, HDLC ELISA KIT
Packing:96T

Catalog No.:ELI-03237h

Gene Name:HDLC

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:HDL-cholesterol(HDLC

Alternative Name:HDLC,HDL-cholesterol(HDLC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human HDL- cholesterol, HDLC ELISA KIT allows for the in vitro quantitative determination of HDL-cholesterol(HDLC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human HDL- cholesterol, HDLC ELISA KIT has been pre-coated with an HDL-cholesterol(HDLC antibody specific to HDL-cholesterol(HDLC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HDL-cholesterol(HDLC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HDL-cholesterol(HDLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of HDL-cholesterol(HDLC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!