Human Heat shock 70 kDa protein 12A,HSPA12A ELISA KIT

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  • Model: ELI-30984h
  • 20 Units in Stock
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Human HSPA12A ELISA Kit


FOR RESEARCH USE ONLY!

96 Tests

 

Alternative name

Human HSPA12A ELISA KIT ,Human heat shock 70 kDa protein 12A ELISA KIT ,Human heat shock 70kD protein 12A ELISA KIT ,Human heat shock 70kDa protein 12A ELISA KIT ,Human heat shock protein family A (Hsp70) member 12A ELISA KIT

 

Intended use

Human HSPA12A ELISA kit allows for the in vitro quantitative determination of Human HSPA12A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

Human HSPA12A ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSPA12A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HSPA12A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HSPA12A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Human HSPA12A ELISA kit  Range 0.32-20ng/ml

 

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