Human heme oxygenase 2 ELISA Kit

Starting at: $696.00

  • Model: ELA-E0589h
  • 20 Units in Stock
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Human Heme oxygenase 2, HO-2 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Heme oxygenase 2,HMOX2,HO-2,HO2,heme oxygenase 2; heme oxygenase (decycling) 2

 

Search name

Human Heme oxygenase 2 ELISA KIT ,Human HMOX2 ELISA KIT ,Human HO-2 ELISA KIT ,Human HO2 ELISA KIT ,Human heme oxygenase 2 ELISA KIT ,Human heme oxygenase (decycling) 2 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human heme oxygenase 2,HO-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HO-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HO-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HO-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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