Human Hemoglobin subunit alpha,HBA ELISA Kit

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  • Model: ELA-E05171h
  • 20 Units in Stock
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Human Hemoglobin subunit alpha, HBA ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Hemoglobin subunit alpha,Alpha-globin,Hemoglobin alpha chain,HBA1,hemoglobin subunit alpha; HBH; alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; alpha-globin; delta globin; hemoglobin alpha 1 globin chain; hemoglobin alpha chain; hemoglobin alpha-1 chain; hemoglobin, alpha 1

 

Search name

Human Hemoglobin subunit alpha ELISA KIT ,Human Alpha-globin ELISA KIT ,Human Hemoglobin alpha chain ELISA KIT ,Human HBA1 ELISA KIT ,Human hemoglobin subunit alpha ELISA KIT ,Human HBH ELISA KIT ,Human alpha one globin ELISA KIT ,Human alpha-1 globin ELISA KIT ,Human alpha-1-globin ELISA KIT ,Human alpha-2 globin chain ELISA KIT ,Human alpha-globin ELISA KIT ,Human delta globin ELISA KIT ,Human hemoglobin alpha 1 globin chain ELISA KIT ,Human hemoglobin alpha chain ELISA KIT ,Human hemoglobin alpha-1 chain ELISA KIT ,Human hemoglobin alpha 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Hemoglobin subunit alpha concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin subunit alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hemoglobin subunit alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hemoglobin subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hemoglobin subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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