Human HIF-3-alpha,HIF3A ELISA Kit

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  • Model: ELI-48650h
  • 20 Units in Stock
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Human HIF-3-alpha, HIF3A ELISA Kit

FOR RESEARCH USE ONLY

96 Tests

 

Alternative name

HIF3A,HIF-3A; IPAS; MOP7; PASD7; bHLHe17; HIF3-alpha-1; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7; class E basic helix-loop-helix protein 17; hypoxia inducible factor 3 alpha subunit; inhibitory PAS domain protein; member of PAS protein 7; hypoxia inducible factor 3 alpha subunit, Hypoxia-inducible factor 3-alpha,HIF-3-alpha,HIF3-alpha, Inhibitory PAS domain protein,HIF3-alpha-1

 

Search name

Human HIF3A ELISA KIT ,Human HIF-3A ELISA KIT ,Human IPAS ELISA KIT ,Human MOP7 ELISA KIT ,Human PASD7 ELISA KIT ,Human bHLHe17 ELISA KIT ,Human HIF3-alpha-1 ELISA KIT ,Human PAS domain-containing protein 7 ELISA KIT ,Human basic-helix-loop-helix-PAS protein MOP7 ELISA KIT ,Human class E basic helix-loop-helix protein 17 ELISA KIT ,Human hypoxia inducible factor 3 alpha subunit ELISA KIT ,Human inhibitory PAS domain protein ELISA KIT ,Human member of PAS protein 7 ELISA KIT ,Human hypoxia inducible factor 3 alpha subunit ELISA KIT ,Human Hypoxia-inducible factor 3-alpha ELISA KIT ,Human HIF-3-alpha ELISA KIT ,Human HIF3-alpha ELISA KIT ,Human Inhibitory PAS domain protein ELISA KIT ,Human HIF3-alpha-1 ELISA KIT

 

Intended use

Human HIF3A kit allows for the in vitro quantitative determination of human HIF-3-alpha concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Human HIF3A kit has been pre-coated with an antibody specific to HIF-3-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHIF-3-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HIF-3-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HIF-3-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 78.0 -5000pg/ml

PDF Manual download

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