Human HK3 ELISA Kit

Product Name:Human HK3 ELISA Kit
Packing:96T

Catalog No.:ELA-E1746h

Gene Name:HK3

Detect Range:0.156-10 ng/mL

Sensitivity:0.076ng/mL

Target Protein Name:Human Hexokinase-3

Alternative Name:Hexokinase-3, Hexokinase type III, HK III, HK3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human HK3 ELISA Kit allows for the in vitro quantitative determination of Human Hexokinase-3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human HK3 ELISA Kit has been pre-coated with an HK3 antibody specific to Human Hexokinase-3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Hexokinase-3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Hexokinase-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm