Human HMGCS1 ELISA Kit

Product Name:Human HMGCS1 ELISA Kit
Packing:96T

Catalog No.:ELA-E1759h

Gene Name:HMGCS1

Detect Range:0.156-10 ng/mL

Sensitivity:0.053 ng/mL

Target Protein Name:Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Alternative Name:Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS1, HMGCS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human HMGCS1 ELISA Kit allows for the in vitro quantitative determination of Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human HMGCS1 ELISA Kit has been pre-coated with an HMGCS1 antibody specific to Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!