Human Hyaluronic acid ELISA Kit

Starting at: $696.00

  • Model: ELA-E0182h
  • 20 Units in Stock
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Human Hyaluronic acid, HA ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Hyaluronic acid,Hyaluronan,Hyaluronate,;

Search name
Human Hyaluronic acid ELISA Kit, Human HA ELISA Kit,Hyaluronic acid ELISA Kit,HA ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general hyaluronic acid,Hyaluronic acid concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principleThis assay employs a two-site sandwich ELISA to quantitate HA in Human serum, plasma. An antibody specific for HA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HA bound in the initial step. The color development is stopped and the intensity of the color is measured.

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