Human Hydroxyproline ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Hydroxyproline,HYP

Search name
Human Hydroxyproline ELISA Kit, Human Hyp ELISA Kit, Hydroxyproline ELISA Kit, Hyp ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general hydroxyproline,Hydroxyproline concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Hydroxyproline, During the reaction, Hydroxyproline in the sample or standard competes with a
fixed amount of biotin-labeled Hydroxyproline for sites on a pre-coated Monoclonal antibody specific to Hydroxyproline. Excess conjugate
and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Hydroxyproline in the samples is then determined by comparing the O.D. of the samples to the standard curve.