Human Immunity-related GTPase family M protein,IRGM ELISA KIT

Starting at: $696.00

  • Model: ELI-48787h
  • 20 Units in Stock
Ask a question


Please Choose:


Add to Cart:

Human IRGM ELISA KIT

Packing         96tests                                                                         Manual

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

 

Gene Name  IRGM

Protein Name  Immunity-related GTPase family M protein
 

Intended use

Human IRGM ELISA KIT allows for the in vitro quantitative determination of IRGM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

REAGENTS AND MATERIALS PROVIDED

Assay plate (96 Wells)    1                                           CoA    1
Standard (lyophilized)    2                                           Sample Diluent    1 x 20 mL
Biotin-Conjugate (concentrate 100 x)    1  x 120μL    Biotin-Conjugate Diluent    1 x 20 mL
Streptavidin-HRP (concentrate 100 x)    1 x 120μL     Streptavidin-HRP Diluent    1 x 20 mL
Wash Buffer (concentrate 25 x)    1 x 20 mL               Substrate Solution    1 x 12 mL
Stop Solution    1 x 10 mL                                           Adhesive Films    3
 

 

Test principle      

The microtiter plate provided in Human IRGM ELISA KIT has been pre-coated with an IRGM antibody specific to IRGM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IRGM and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IRGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IRGM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Introduction

Members of the p47 immunity-related GTPase (IRG) family, including IRGM, play an important role in the murine immune system; however, in humans this resistance system is greatly reduced. There is evidence that human IRGM plays a role in autophagy and control of intracellular mycobacteria . In mouse, Irgm belongs to a family of gamma-interferon (IFNG)-induced 4 GTP-binding proteins of approximately 47 to 48 kD. Murine Irgm1 induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. They also identified a function for human IRGM in the control of intracellular pathogens and reported that human IRGM plays a role in autophagy and in the reduction of intracellular bacillary load.Members of the p47 immunity-related GTPase (IRG) family, including IRGM, play an important role in the murine immune system; however, in humans this resistance system is greatly reduced. There is evidence that human IRGM plays a role in autophagy and control of intracellular mycobacteria . In mouse, Irgm belongs to a family of gamma-interferon (IFNG)-induced 4 GTP-binding proteins of approximately 47 to 48 kD. Murine Irgm1 induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. They also identified a function for human IRGM in the control of intracellular pathogens and reported that human IRGM plays a role in autophagy and in the reduction of intracellular bacillary load.

 

Caution:
1.  This product is FOR RESEARCH USE ONLY!

 

 

 

Alternative Name

Human IRGM ELISA KIT ,Human LRG 47 ELISA KIT ,Human Ifggd3 ELISA KIT ,Human Ifi1 ELISA KIT ,Human Iigp3 ELISA KIT ,Human Iipg3 ELISA KIT ,Human immunity-related GTPase family M protein 1 ELISA KIT ,Human LPS-stimulated RAW 264.7 macrophage protein 47 ELISA KIT ,Human immunity related GTPase m1 ELISA KIT ,Human interferon-gamma-inducible GTPase Ifggd3 protein ELISA KIT ,Human interferon-inducible protein 1 ELISA KIT ,Human immunity-related GTPase family M member 1 ELISA KIT

No customer comments for the moment.

Add A Comment

Related Products

Cart  

No products

Total $0.00

Prices don't include postage.

Cart