Human Interleukin- 21, IL21 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Interleukin-21,IL-21,Za11, IL21,IL 21,IL-21; Za11; interleukin-21; interleukin-21 isoform; interleukin 21

Search name

Human IL21 ELISA KIT ,Human Interleukin-21 ELISA KIT ,Human Za11 ELISA KIT ,Human  IL-21 ELISA KIT ,Human IL 21 ELISA KIT ,Human IL-21 ELISA KIT ,Human Za11 ELISA KIT ,Human interleukin-21 ELISA KIT ,Human interleukin-21 isoform ELISA KIT ,Human interleukin 21 ELISA KIT

Intended use

Human IL21 ELISA KIT allows for the in vitro quantitative determination of human interleukin-21,IL-21 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human IL21 ELISA KIT has been pre-coated with an antibody specific to IL-21. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-21 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human IL21 ELISA KIT Range 15.6-1000pg/ml

Human IL21 ELISA KIT PDF Manual download

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