Human Interleukin- 31, IL31 ELISA KIT

Product Name:Human Interleukin- 31, IL31 ELISA KIT
Packing:96T

Catalog No.:ELI-02775h

Gene Name:IL31

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Interleukin-31(IL31

Alternative Name:IL31,Interleukin-31(IL31
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Interleukin- 31, IL31 ELISA KIT allows for the in vitro quantitative determination of Interleukin-31(IL31 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Interleukin- 31, IL31 ELISA KIT has been pre-coated with an Interleukin-31(IL31 antibody specific to Interleukin-31(IL31 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Interleukin-31(IL31 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Interleukin-31(IL31, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Interleukin-31(IL31 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!