Human IQ domain- containing protein H, IQCH ELISA KIT
Product Name:Human IQ domain- containing protein H, IQCH ELISA KIT
Packing:96T
Catalog No.:ELI-21129h
Gene Name:IQCH
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:IQ domain-containing protein H(IQCH
Alternative Name:IQCH,IQ domain-containing protein H(IQCH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IQ domain- containing protein H, IQCH ELISA KIT allows for the in vitro quantitative determination of IQ domain-containing protein H(IQCH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IQ domain- containing protein H, IQCH ELISA KIT has been pre-coated with an IQ domain-containing protein H(IQCH antibody specific to IQ domain-containing protein H(IQCH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IQ domain-containing protein H(IQCH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IQ domain-containing protein H(IQCH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of IQ domain-containing protein H(IQCH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!