Human ITGAV ELISA Kit
Product Name:Human ITGAV ELISA Kit
Packing:96T
Catalog No.:ELA-E0271h
Gene Name:ITGAV
Detect Range:0.312-20 ng/mL
Sensitivity:0.11ng/mL
Target Protein Name:Human Integrin alpha-V
Alternative Name:Integrin alpha-V, Vitronectin receptor subunit alpha, ITGAV, MSK8, VNRA, CD51
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ITGAV ELISA Kit allows for the in vitro quantitative determination of Human Integrin alpha-V concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ITGAV ELISA Kit has been pre-coated with an ITGAV antibody specific to Human Integrin alpha-V .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Integrin alpha-V and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Integrin alpha-V, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm