Human Keratin 20 ELISA Kit

Product Name:Human Keratin 20 ELISA Kit
Packing:96T

Catalog No.:ELA-E9127h

Gene Name:KRT20

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Keratin 20

Alternative Name:KRT20,Keratin 20
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Keratin 20 ELISA Kit allows for the in vitro quantitative determination of Keratin 20 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Keratin 20 ELISA Kit has been pre-coated with an Keratin 20 antibody specific to Keratin 20 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Keratin 20 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Keratin 20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Keratin 20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!