Human Kinesin- like protein KIF3A, KIF3A ELISA KIT
Product Name:Human Kinesin- like protein KIF3A, KIF3A ELISA KIT
Packing:96T
Catalog No.:ELI-14147h
Gene Name:KIF3A
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Kinesin-like protein KIF3A(KIF3A
Alternative Name:KIF3A,Kinesin-like protein KIF3A(KIF3A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Kinesin- like protein KIF3A, KIF3A ELISA KIT allows for the in vitro quantitative determination of Kinesin-like protein KIF3A(KIF3A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Kinesin- like protein KIF3A, KIF3A ELISA KIT has been pre-coated with an Kinesin-like protein KIF3A(KIF3A antibody specific to Kinesin-like protein KIF3A(KIF3A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kinesin-like protein KIF3A(KIF3A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kinesin-like protein KIF3A(KIF3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Kinesin-like protein KIF3A(KIF3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!