Human Kininogen-1, KNG1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Kininogen-1,Kininogen I,Thiol proteinase inhibitor, KNG1,kininogen-1; BDK; KNG; HMWK; alpha-2-thiol proteinase inhibitor; bradykinin; fitzgerald factor; high molecular weight kininogen; williams-Fitzgerald-Flaujeac factor; kininogen 1
Search name
Human Kininogen-1 ELISA KIT ,Human Kininogen I ELISA KIT ,Human Thiol proteinase inhibitor ELISA KIT ,Human KNG1 ELISA KIT ,Human kininogen-1 ELISA KIT ,Human BDK ELISA KIT ,Human KNG ELISA KIT ,Human HMWK ELISA KIT ,Human alpha-2-thiol proteinase inhibitor ELISA KIT ,Human bradykinin ELISA KIT ,Human fitzgerald factor ELISA KIT ,Human high molecular weight kininogen ELISA KIT ,Human williams-Fitzgerald-Flaujeac factor ELISA KIT ,Human kininogen 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Kininogen-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kininogen-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Kininogen-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kininogen-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Kininogen-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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