Human KL ELISA Kit
Product Name:Human KL ELISA Kit
Packing:96T
Catalog No.:ELA-E2253h
Gene Name:KL
Detect Range:7.8-500 pg/mL
Sensitivity:2.4 pg/mL
Target Protein Name:Human Klotho
Alternative Name:Klotho, KL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human KL ELISA Kit allows for the in vitro quantitative determination of Human Klotho concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human KL ELISA Kit has been pre-coated with an KL antibody specific to Human Klotho .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Klotho and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Klotho, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Klotho in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!