Human LAP3 ELISA KIT

$696.00

  • Model: EH06618
  • 100 Units in Stock
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Human LAP3 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  LAP3

Protein Name  Cytosol aminopeptidase

Alternative Name

Human LAP3 ELISA KIT ,Human HEL-S-106 ELISA KIT ,Human LAP ELISA KIT ,Human LAPEP ELISA KIT ,Human PEPS ELISA KIT ,Human cytosol aminopeptidase ELISA KIT ,Human LAP-3 ELISA KIT ,Human epididymis secretory protein Li 106 ELISA KIT ,Human leucyl aminopeptidase ELISA KIT ,Human peptidase S ELISA KIT ,Human proline aminopeptidase ELISA KIT ,Human prolyl aminopeptidase ELISA KIT ,Human leucine aminopeptidase 3 ELISA KIT

Human LAP3 ELISA KIT Range  0.156-10ng/mL

Intended use

Human LAP3 ELISA KIT allows for the in vitro quantitative determination of LAP3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Human LAP3 ELISA KIT has been pre-coated with an LAP3 antibody specific to LAP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LAP3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LAP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LAP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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