Human leukocyte common antigen ELISA Kit

Product Name:Human leukocyte common antigen ELISA Kit
Packing:96T

Catalog No.:ELA-E1030h

Gene Name:LCA(CD45RB

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:leukocyte common antigen

Alternative Name:LCA(CD45RB,leukocyte common antigen
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human leukocyte common antigen ELISA Kit allows for the in vitro quantitative determination of leukocyte common antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human leukocyte common antigen ELISA Kit has been pre-coated with an leukocyte common antigen antibody specific to leukocyte common antigen .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for leukocyte common antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain leukocyte common antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of leukocyte common antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!