Human low density lipoprotein ELISA Kit

Product Name:Human low density lipoprotein ELISA Kit
Packing:96T

Catalog No.:ELA-E1107h

Gene Name:LDL

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:low density lipoprotein

Alternative Name:LDL,low density lipoprotein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human low density lipoprotein ELISA Kit allows for the in vitro quantitative determination of low density lipoprotein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human low density lipoprotein ELISA Kit has been pre-coated with an low density lipoprotein antibody specific to low density lipoprotein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for low density lipoprotein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain low density lipoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of low density lipoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!