Human MAPK14 ELISA Kit

Product Name:Human MAPK14 ELISA Kit
Packing:96T

Catalog No.:ELA-E9114h

Gene Name:MAPK14

Detect Range:0.156-10 ng/mL

Sensitivity:0.098ng/mL

Target Protein Name:Human Mitogen-activated protein kinase 14

Alternative Name:Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, CSBP, MAP kinase MXI2, MAX-interacting
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human MAPK14 ELISA Kit allows for the in vitro quantitative determination of Human Mitogen-activated protein kinase 14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human MAPK14 ELISA Kit has been pre-coated with an MAPK14 antibody specific to Human Mitogen-activated protein kinase 14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Mitogen-activated protein kinase 14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Mitogen-activated protein kinase 14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Mitogen-activated protein kinase 14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!