Human Melanocortin receptor 4,MC4R ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Melanocortin receptor 4, MC4R,melanocortin 4 receptor,MC4-R

Search name

Human Melanocortin receptor 4 ELISA KIT ,Human  MC4R ELISA KIT ,Human melanocortin 4 receptor ELISA KIT ,Human MC4-R ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human melanocortin receptor 4,MC4-R concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MC4-R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MC4-R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MC4-R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MC4-R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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