Human Metabotropic glutamate receptor 3, GRM3 ELISA KIT

Product Name:Human Metabotropic glutamate receptor 3, GRM3 ELISA KIT
Packing:96T

Catalog No.:ELI-27893h

Gene Name:GRM3

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Metabotropic glutamate receptor 3(GRM3

Alternative Name:GRM3,Metabotropic glutamate receptor 3(GRM3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Metabotropic glutamate receptor 3, GRM3 ELISA KIT allows for the in vitro quantitative determination of Metabotropic glutamate receptor 3(GRM3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Metabotropic glutamate receptor 3, GRM3 ELISA KIT has been pre-coated with an Metabotropic glutamate receptor 3(GRM3 antibody specific to Metabotropic glutamate receptor 3(GRM3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Metabotropic glutamate receptor 3(GRM3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Metabotropic glutamate receptor 3(GRM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Metabotropic glutamate receptor 3(GRM3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!