Human Methionine adenosyltransferase 2,MAT 2 ELISA Kit

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  • Model: ELA-60006h
  • 20 Units in Stock
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Human Methionine adenosyltransferase 2, MAT 2 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
S-adenosylmethionine synthase isoform type-2(AdoMet synthase 2),Methionine adenosyltransferase 2(MAT 2),Methionine
adenosyltransferase II(MAT-II), MAT2A,adoMet synthase 2; MATA2; MATII; SAMS2; MAT 2; MAT-II; S-adenosylmethionine synthase
isoform type-2; adoMet synthetase 2; methionine adenosyltransferase 2; methionine adenosyltransferase II, alpha

Search name
Human S-adenosylmethionine synthase isoform type-2 ELISA Kit, Human AdoMet synthase 2 ELISA Kit, Human MAT 2 ELISA
Kit, Human Methionine adenosyltransferase 2 ELISA Kit, Human Methionine adenosyltransferase II ELISA Kit, Human MAT-II
ELISA Kit, Human MAT2A ELISA Kit, Human MATA2 ELISA Kit, Human MATII ELISA Kit, Human SAMS2 ELISA Kit, Human
methionine adenosyltransferase II alpha ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human s-adenosylmethionine synthase isoform type-2,AdoMet
synthase 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AdoMet synthase 2. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AdoMet synthase
2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain AdoMet synthase 2, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AdoMet synthase 2 in the samples is
then determined by comparing the O.D. of the samples to the standard curve.

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