Human MORN repeat- containing protein 4, MORN4 ELISA KIT

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  • Model: ELI-14128h
  • 20 Units in Stock
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Human MORN4 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  MORN4

Protein Name  MORN repeat-containing protein 4

Alternative Name

Human MORN4 ELISA KIT, Human C10orf83 ELISA KIT ,Human UTA ELISA KIT ,Human bA548K23.4 ELISA KIT ,Human rtp ELISA KIT ,Human MORN repeat-containing protein 4 ELISA KIT ,Human 44050 protein ELISA KIT ,Human protein 44050 ELISA KIT ,Human retinophilin homolog ELISA KIT ,Human MORN repeat containing 4 ELISA KIT

 

Intended use

Human MORN4 ELISA KIT allows for the in vitro quantitative determination of MORN4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Human MORN4 ELISA KIT has been pre-coated with an MORN4 antibody specific to MORN4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MORN4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MORN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MORN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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