Human Myo Inositol Oxygenase, MIOX ELISA kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

nositol oxygenase,Renal-specific oxidoreductase,Myo-inositol oxygenase,MI oxygenase,Aldehyde reductase-like 6,Kidney-specific protein 32, MIOX,myo inositol oxygenase,ALDRL6; MI oxygenase; aldehyde reductase (aldose reductase) like 6; aldehyde reductase-like 6; inositol oxygenase; kidney-specific protein 32; renal-specific oxidoreductase; myo-inositol oxygenase

Search name

Human nositol oxygenase ELISA KIT ,Human Renal-specific oxidoreductase ELISA KIT ,Human Myo-inositol oxygenase ELISA KIT ,Human MI oxygenase ELISA KIT ,Human Aldehyde reductase-like 6 ELISA KIT ,Human Kidney-specific protein 32 ELISA KIT ,Human MIOX ELISA KIT ,Human myo inositol oxygenase ELISA KIT ,Human ALDRL6 ELISA KIT ,Human MI oxygenase ELISA KIT ,Human aldehyde reductase (aldose reductase) like 6 ELISA KIT ,Human aldehyde reductase-like 6 ELISA KIT ,Human inositol oxygenase ELISA KIT ,Human kidney-specific protein 32 ELISA KIT ,Human renal-specific oxidoreductase ELISA KIT ,Human myo-inositol oxygenase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Inositol oxygenase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol oxygenase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Inositol oxygenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inositol oxygenase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inositol oxygenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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