Human Nebulette, NEBL ELISA KIT

Product Name:Human Nebulette, NEBL ELISA KIT
Packing:96T

Catalog No.:ELI-05377h

Gene Name:NEBL

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Nebulette(NEBL

Alternative Name:NEBL,Nebulette(NEBL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Nebulette, NEBL ELISA KIT allows for the in vitro quantitative determination of Nebulette(NEBL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Nebulette, NEBL ELISA KIT has been pre-coated with an Nebulette(NEBL antibody specific to Nebulette(NEBL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Nebulette(NEBL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nebulette(NEBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Nebulette(NEBL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!