Human Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein, Nos1ap ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human NOS1AP ELISA KIT ,Human CAPON ELISA KIT ,Human 6330408P19Rik ELISA KIT ,Human C-terminal PDZ domain ligand of neuronal nitric oxide synthase ELISA KIT ,Human C-terminal PDZ ligand of neuronal nitric oxide synthase protein ELISA KIT ,Human ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain ELISA KIT ,Human nitric oxide synthase 1 (neuronal) adaptor protein ELISA KIT ,Human nitric oxide synthase 1 adaptor protein ELISA KIT

Intended use

Human Nos1ap ELISA kit allows for the in vitro quantitative determination of Human Nos1ap concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human Nos1ap ELISA kit has been pre-coated with an antibody specific to Nos1ap. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Nos1ap and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nos1ap, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Nos1ap in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.31-20ng/ml

Human Nos1ap ELISA kit manual download

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