• Model: EF007364
  • 100 Units in Stock
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Catalog  EF007364

Packing 48T/96T

Reactivity Human

Detect Range 31.25-2000pg/ml

NPTN ELISA KIT Sensitivity< 18.75pg/ml

Application NPTN ELISA KIT|Human  is for detecting the concentration of Human NPTN in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade


Principle of NPTN ELISA KIT|Human

The microtiter plate provided in NPTN ELISA KIT|Human has been pre-coated with an Human NPTN antibody specific to Human NPTN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human NPTNantibody preparation specific for Human NPTN then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human NPTNin the samples is then determined by comparing the O.D. of the samples to the standard curve.


*Please noted that NPTN ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!

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