Human NUDT15 ELISA KIT

Starting at: $696.00

  • Model: ELI-13382h
  • 20 Units in Stock
Ask a question


Please Choose:


Add to Cart:

Human NUDT15 ELISA KIT

Product Name:Human NUDT15 ELISA KIT
Packing:96T                                                                                Manual

Catalog No.:ELI-13382h

Gene Name:NUDT15

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Human NUDT15

Alternative Name:NUDT15,Human NUDT15
Human NUDT15 INTRODUCTION: May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP (PubMed:26238318). Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives (PubMed:22556419, PubMed:26238318). Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear (PubMed:26238318, PubMed:22556419). Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs (PubMed:26238318, PubMed:25108385). May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA (PubMed:19419956).


Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type: Sandwich ELISA Kit
Product Description:Human NUDT15 ELISA KIT allows for the in vitro quantitative determination of Human NUDT15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human NUDT15 ELISA KIT has been pre-coated with an Human NUDT15 antibody specific to Human NUDT15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human NUDT15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NUDT15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human NUDT15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

No customer comments for the moment.

Add A Comment

Related Products

Cart  

No products

Total $0.00

Prices don't include postage.

Cart