Human Occludin, OCLN ELISA KIT

Product Name:Human Occludin, OCLN ELISA KIT
Packing:96T

Catalog No.:ELI-21396h

Gene Name:OCLN

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Occludin(OCLN

Alternative Name:OCLN,Occludin(OCLN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Occludin, OCLN ELISA KIT allows for the in vitro quantitative determination of Occludin(OCLN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Occludin, OCLN ELISA KIT has been pre-coated with an Occludin(OCLN antibody specific to Occludin(OCLN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Occludin(OCLN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Occludin(OCLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Occludin(OCLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!